Biocompatibility of polyhydroxybutyrate microspheres: in vitro and in vivo evaluation.
نویسندگان
چکیده
Microspheres have been prepared from the resorbable linear polyester of beta-hydroxybutyric acid (polyhydroxybutyrate, PHB) by the solvent evaporation technique and investigated in vitro and in vivo. Biocompatibility of the microspheres has been proved in tests in the culture of mouse fibroblast cell line NIH 3T3 and in experiments on intramuscular implantation of the microspheres to Wistar rats for 3 months. Tissue response to the implantation of polymeric microspheres has been found to consist in a mild inflammatory reaction, pronounced macrophage infiltration that increases over time, involving mono- and poly-nuclear foreign body giant cells that resorb the polymeric matrix. No fibrous capsules were formed around polymeric microparticles; neither necrosis nor any other adverse morphological changes and tissue transformation in response to the implantation of the PHB microparticles were recorded. The results of the study suggest that polyhydroxybutyrate is a good candidate for fabricating prolonged-action drugs in the form of microparticles intended for intramuscular injection.
منابع مشابه
Formulation and Evaluation of Propranolol Hydrochloride-Loaded Carbopol-934P/Ethyl Cellulose Mucoadhesive Microspheres
The purpose of this research was to formulate and systemically evaluate in-vitro and in-vivo performances of mucoadhesive propranolol hydrochloride microspheres for its potential use in the treatment of hypertension, myocardial infraction and cardiac arrhythmias. Propranolol hydrochloride mucoadhesive microspheres, containing carbopol-934P as mucoadhesive polymer and ethyl cellulose as carrier ...
متن کاملFormulation and Evaluation of Propranolol Hydrochloride-Loaded Carbopol-934P/Ethyl Cellulose Mucoadhesive Microspheres
The purpose of this research was to formulate and systemically evaluate in-vitro and in-vivo performances of mucoadhesive propranolol hydrochloride microspheres for its potential use in the treatment of hypertension, myocardial infraction and cardiac arrhythmias. Propranolol hydrochloride mucoadhesive microspheres, containing carbopol-934P as mucoadhesive polymer and ethyl cellulose as carrier ...
متن کاملFormulation and In Vitro Evaluation of Acyclovir Mucoadhesive Microspheres for Intravaginal Application
The purpose of the research was to formulate microspheres of acyclovir (ACV) using mucoadhesive polymers, sodium alginate and chitosan. Calcium chloride was used as the ionotropic gelling agent. Sodium alginate was crosslinked by calcium chloride leading to a slower release of the drug. Chitosan which is a cationic polymer interacted with sodium alginate, an anionic polymer, to form an interpol...
متن کاملEvaluation of Biopolymers Effect on In vitro Biocompatibility Property of Nano Hydroxyl Apatite Composites
In this work, we report the effect of biopolymers (starch and gelatin) on in vitro biocompatibility property of nano hydroxyapatite (nHAp) composites. Cell culture and MTT assays were performed for in vitro biocompatibility. They show that nHAp can affect the proliferation of cells and the nHAp-starch and nHAP-gelatin biocomposites have no negative effect on the cell morphology, viability and p...
متن کاملBiodegradable Multiblock Copolymers for Drug Delivery Applications
Microspheres of a hydrophobic and a hydrophilic poly(ether-ester) copolymer were evaluated for their in-vitro and in-vivo biocompatibility and degradation. The microspheres prior to and after sterilization were tested for in-vitro cytotoxicity. The invivo biocompatibility of the poly(ethylene glycol) terephthalate and poly(butylene terephthalate) (PEGT/PBT) microspheres was evaluated subcutaneo...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Journal of materials science. Materials in medicine
دوره 19 6 شماره
صفحات -
تاریخ انتشار 2008